National Centers for Translational Research in Reproduction and Infertility

Center For Human Embryonic Stem Cell Research

Stanford University

The objective of our Center is to promote scientific excellence in translational science via well-designed studies of human germ cell development, based on a foundation of knowledge in model systems, and culminating with applications that address critical clinical need. To accomplish our objective, we propose three projects, a pilot project, and four cores. We also present several opportunities for a translational pilot project.

Project 1 (Dr. R.A. Reijo Pera, PI): Germ Cell Differentiation from Human iPSCs and hESCs

Project 2 (Dr. M.T. Fuller, PI): Translational Regulation of Meiotic Cell Cycle Onset and Progression in the Male

Project 3 (Dr. A. Hsueh, PI): Derivation of Mature Human Oocytes from Primordial Follicles

Pilot Project (Dr. V. Sebastiano, PI): Regulation of Translational Control in the Oocyte to Embryo Transition

Supporting the research are four cores: A) Administration; B) Outreach and Education; C) Microanalysis, Sequencing and Informatics; D) Reproductive Database. This application is a culmination of our reorganization and planning over the last several years and presents our vision for Reproductive and Stem Cell Biology based on outstanding basic and translational science. The application is put forth by a collaborative team that shares common interests in terms of genes and germ cell differentiation and maturation, pathways, developmental systems and overall educational, outreach and research goals. Each project consists of a strong basic component; in addition, three of the five projects and pilots have an equally-strong clinical component encompassing genetic analysis of human germ cell development from pluripotent stem cells to probe fundamental aspects and potential therapies, deriving mature oocytes to remedy primary ovarian insufficiency, and establishing the first registry of women with POI. Each project is relevant to the health of infertile women and men and each is informed by the elegant genetic systems of Drosophila and the mouse. The central theme of our Center is novel, forward-looking and built on a firm foundation of scientific and clinical inquiry with an innovative outreach and educational component with hopes of reaching out to other scientists, healthcare professionals.